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KMID : 0357319950300060718
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Journal of the Korean Society for Microbiology
1995 Volume.30 No. 6 p.718 ~ p.726
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PCR-RFLP Analysis for Detection Point Mutations at 12th Codon of Oncogene Ras in Korean Colorectal Cancers
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Abstract
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The point mutations of ras oncogene in human tumor have been well documented. These mutations have been exclusively localized to codon 12, 13 and 61 of three ras genes(K-, H-, N-ras). We have evaluated the point mutation of c-K-ras and c-Ha-ras
codon 12
in Korean colorectal cancers using recently developed PCR(Polymerase Chain Reaction) - RFLP(Restriction Fragment Length Polymorphism) method.
The presence of mutations at codon 12 of c-Ha-ras and c-K-ras gene was examined by the endogeneous or primer mediated restriction fragment length polymorphism using polymerase chain reaction. Five of 30 colorectal cancers tested contained
mutations
at
codon 12 of c-K-ras gene. On the other hand, no mutations were found at codon 12 of c-Ha-ras gene. The mutations were confirmed by PCR product cloning and nucleic acid sequencing. We think that PCR-RFLP method is accurate and time saving, and can
be
applied toward the rapid, non-radioactive screening of colorectal cancers.
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